AGRICULTURE

Our product QL-AGRI, commercialized by BASF has several applications:

1. Natural nematode control

QL-AGRI has shown to be effective in controlling nematode population in citrus plantations and vineyards. QL-AGRI controls fitoparasite nematodes such as Meloidogine with similar efficiency as that obtained with chemical nematicides (e.g. fenamifos-NEMACUR, Bayer; etoprofos- MOCAP, Rhône Poulenc).

It appears that the nematicide effect in QL-AGRI is a result of saponins binding to plant sterol components, inhibiting nematode cholesterol synthesis, essential for nematode growth and survival. Independent research done by Natural Response show the there is a synergistic effect between saponins and other active compounds present in quillaja extracts such as polyphenols. These results are consistent with scientific literature that informs of other plants rich in saponins and tannins capable of controlling nematodes.

2. Natural fungi control

QL-AGRI may help in fungi control, especially in integrated pest management systems. Saponins present in QL-AGRI form complexes with cell wall sterols of fungi producing a “microporation” which induces cell lysis.

Our research conducted in-vitro and on field trials shows that QL-AGRI controls fungi such as Erysiphe cichoracearum (oidium) that cause severe crop damage, using a dose of 1- 5 kg QL-AGRI / hectare. The product is diluted to a concentration of 200 – 300 ppm and applied by aspersion.

3. Natural bio repellent

The addition of QL-AGRI in combination with other natural products like cimanic aldehyde, has been proven to be an effective repellent for pests such as fungi, insects, arachnids, aphids, etc.

QL-AGRI may also be used in combination with capsicum oleoresin in polymeric paints for minimizing the presence of birds, rodents, mollusks and crustaceae. In combination with Yucca extract it is very effective against snails.

4. Plant growth stimulation

Research performed in the Investigations done in 1940 by the Société de Biologie de Marseille, France(1) showed that the germination rate of certain seeds increases with the use of quillaja extracts (equivalent to 100 ppm pf QL-AGRI). An increase in the vegetative growth was also observed in plants treated with quillaja extracts once or twice a week this has been confirmed lately in radish.

These effects seem to be related to a better transfer of nutrients of the plant due to changes in the cell wall permeability by saponins. However, at times growth is accelerated in such a way that other saponin related mechanisms are probably involved. In these cases, the effect is comparable to plant growth hormones (e.g. IAA).

In field trials greater growing rates have been observed in tomatoes, cucumbers and grapes at a dosage of only 25 –30 lt QL-AGRI / hectare rendering crops 20 to 30% greater than that of control groups with no QL-AGRI. Positive effects have also been observed in some flower species.

5. Natural surfactant / wetting agent

Most pesticides commonly used in agriculture require a wetting agent when applied in order to obtain good leaf wetting rates. Surfactants lower the surface tension of water, allowing it to cover the surface of the leaves more efficiently giving also better adherence of the pesticide to the plant.

QL-AGRI reduces the surface tension of water from 72 dyne/cm, normal value for pure water, to approximately 40 dyne/cm with a dosage of 400 ppm. Surface tension can be lowered even more with the addition of small amounts of synthetic surfactants. This is useful when it is needed to reduce chemical surfactants use in a given application.

LITERATURE

1. Balansard, J. and Pellisier, F. 1943 Les saponines de quillaya et polygala. Agents de proliferation cellulaire Proceeding of the Société de Biologie de Marseille. Meeting May 10: 523-524.

2. Balansard, J. Pellisier and S. Conil. 1945. Action de saponites de Quillaya et de Sapindus sur le pouvoir absorbant, la germination et la croissance de Zea mais Proceedings Société de Biologie de Marseille. Meeting November 28: 140-142.

3. Emerson; Ralph W. ; Crandall, Jr.; Bradford G., 1997. Use of saponin in methods and compositions for pathogen control USP 5,639,794

4. Fukai Katsuhiko, Hara Masahiko, Hashizume Akito,1995. Antiplant Viral Agent JP 7025718A

5. Hewlett; Eric M.; Hewlett; Thomas E., 1997. Compositions and methods for controlling nematodes USP 5,595,748.

6. Lowe, J.A. 2000. Anti-mollusc compositions. WO 00/ 04781

7. Mason; Wenda M. A. Puritch; George S.; Almond; David S., 1994. Saponin containing anti-feedant and molluscicide for terrestrial mollusc control USP 5,290,557

8. Omar, S. A.; Abdel-Massih, M. I.; Mohamed, B. E., 1994. Use of saponin to control the root-knot nematode, Meloidogyne javanica in tomato plants. Bulletin of Faculty of Agriculture, University of Cairo vol. 45 (4): p.933-940

9. Sainz, R. 1999. Uso de extractos de quillay para el control de nemátodos. Memoria. Facultad de Ingeniería, Departamento de Ingeniería Química y Bioprocesos. P. Universidad Católica de Chile.

10. Tashiro Eiichi, 1994. Insect-Proofing Agent For Plant JP 6183925A